BD, 552771, BD Pharmingen™ PerCP-Cy™5.5 Rat Anti-Mouse CD45R/B220

Alternative Name: B220; Ly-5; CD45R; LCA; Ptprc; Protein tyrosine phosphatase receptor type C
Reactivity: Mouse (QC Testing), Human (Tested in Development)
Isotype: Rat IgG2a, κ
Immunogen: Mouse Abelson Leukemia Virus-Induced pre-B tumor cells
Application: Flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_394457
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.