BD, 553435, BD Pharmingen™ Purified Rat Anti-Mouse IgM

Reactivity: Mouse (QC Testing)
Isotype: Rat IgG2a, κ
Immunogen: Not reported
Application: ELISA Capture, Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_394855
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Flow cytometry: For flow cytometric detection of intracytoplasmic IgM, FITC anti-mouse IgM (clone II/41) (Cat. No. 553437) is recommended. ELISA: For detection of mouse IgM by sandwich ELISA, this antibody may be used as the capture antibody at ~ 2 µg/ml coupled with biotin-conjugated rat anti-mouse IgM (clone R6-60.2) (Cat. No. 553406) and avidin-HRP (Cat. No. 554058) as the detection antibody.  Purified mouse IgM (Cat. No. 553472) may be used as the ELISA standard.