BD, 553436, BD Pharmingen™ Biotin Rat Anti-Mouse IgM

Reactivity: Mouse (QC Testing)
Isotype: Rat IgG2a, κ
Immunogen: Not reported
Application: ELISA Detection (Routinely Tested), Flow cytometry (Tested During Development)
Concentration: 0.5 mg/ml
RRID: AB_394856
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures: Recommended Assay Procedures Flow cytometry: For flow cytometric detection of intracytoplasmic IgM, FITC anti-mouse IgM (clone II/41) (Cat. No. 553437) is recommended. ELISA: This antibody, coupled with avidin-HRP (Cat.No. 554058), may be used at ~2 µg/ml as the detection antibody for the detection of mouse IgM by indirect ELISA. For the detection of mouse IgM by sandwich ELISA, purified rat anti-mouse IgM (clone II/41) (Cat. No. 553435) is recommended for capture coupled with  biotin-conjugated rat anti-mouse IgM (clone R6-60.2) (Cat. No. 553406) and avidin-HRP (Cat.No. 554058) for detection.  Purified mouse IgM (Cat.No. 553472) may be used as the ELISA standard for either the indirect or sandwich ELISA.