BD, 553499, BD Pharmingen™ Purified Mouse Anti-Mouse IgG1[a]

Alternative Name: Igh-4a; Ighg1; IgG1
Isotype: Mouse SJL IgG2a, κ
Immunogen: Not reported
Application: ELISA (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_394884
Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Indirect ELISA: Purified mouse IgG1 (a allotype) [MN 557273] may be used as the antigen (e.g coating plates). Purified anti-mouse IgG1[a] allotype specific antibody [MN 553499] may be used at ~ 2 µg/ml followed by biotinylated anti-mouse IgG2a [b] allotype specific antibody [MN 553504] at ~ 2 µg/ml and avidin-HRP [MN 554058] for detection.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.