BD, 553766, BD Pharmingen™ Purified Hamster Anti-Mouse CD80

Alternative Name: Cd80; B7; B7-1; Cd28l; Ly-53; MIC17; TSA1
Reactivity: Mouse (QC Testing), Dog (Reported)
Isotype: Armenian Hamster IgG2, κ
Immunogen: Mouse CD80 (B7) Transfected Cell Line
Application: Flow cytometry (Routinely Tested), Blocking, Immunohistochemistry-frozen, Immunoprecipitation, In vivo exacerbation (Reported)
Concentration: 0.5 mg/ml
RRID: AB_395036
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Precautions for flow cytometry: For flow cytometry of leukocytes, it is recommended that Mouse BD Fc Block™ (Cat. No. 553141) be used.  When using Mouse BD Fc Block™ (clone 2.4G2), it is important that the second-step anti-hamster IgG antibody does not cross-react with the 2.4G2 mAb (rat IgG2b, κ); we have found that the anti-hamster IgG cocktail (Cat. No. 554010) to be effective.  Since this antigen is expressed at low density, it may be desirable to amplify staining by using a biotinylated second-step reagent followed by a "bright" third-step reagent, such as streptavidin-PE (Cat. No. 554061). Caution: Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals.  Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer.  Since endotoxin may also effect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.