BD, 554150, BD Pharmingen™ Purified Mouse Anti-SV40 Large T and Small t Antigens

Reactivity: Viral (QC Testing)
Isotype: Mouse IgG2a
Immunogen: SV40-transformed BALB/c mouse cells
Application: Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
Target Molecular Weight: 90-100 kDa
Concentration: 0.5 mg/ml
RRID: AB_395270
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures By SDS-PAGE, T-ag migrates at 90-100 kDa and t-ag at 15-20 kDa. COS-7 kidney monkey cells (ATCC CRL 1651), other established SV40-transformed cell lines, SV40-virus infected cells, or cells transfected with SV40 DNA should be used as a positive control. Other applications include immunoprecipitation, Western blot analysis, immunofluorescence microscopy, immunohistochemical staining of acetone-fixed, tissue culture cells (cytospin) and SV40 origin DNA binding assays, although this application has not been tested at BD Pharmingen. The biotin-conjugated antibody, Cat. No. 554151 is recommended for cytospin staining and IHC applications.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.