BD, 554284, BD Pharmingen™ Purified Mouse Anti-Human PDGF-AB/BB

Reactivity: Human (QC Testing)
Isotype: Mouse IgG1
Immunogen: Human PDGF BB Recombinant Protein
Application: Western blot (Routinely Tested), Immunoprecipitation (Reported)
Target Molecular Weight: 35-56 kDa
Concentration: 0.5 mg/ml
RRID: AB_395343
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml Note: Investigators should note that due to the ability for this antibody to recognize various PDGF isoforms and/or multimers, bands may be observable migrating in a range between 35-56 kDa (e.g 35 kDa, 42 kDa, and/or 56 kDa). In WI-38 cells (Human lung fibroblasts; ATCC CCL-75), for example, a band may be predominantly observable to migrate ~ 50-56 kDa.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.