BD, 554395, BD Pharmingen™ PE Rat Anti-Mouse/Anti-Human IL-5

Reactivity: Human, Mouse (QC Testing)
Isotype: Rat IgG1, κ
Immunogen: Mouse Semi-Purified T-Cell Clone Supernatant
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_395364
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures Recommended Assay Procedure: For optimal immunofluorescent staining with flow cytometric analysis, this anti-cytokine antibody should be titrated (≤ 0.5 µg mAb/million cells). A suitable rat IgG1 isotype control assessing the level of background staining on fixed/permeabilized or human cells is PE-R3-34 (Cat. No. 554685). An isotype control should be used at comparable concentrations to antibody of interest. For specific methodology, please visit our website protocol section or refer to  Chapter 4: Immunofluorescent Staining of Intracellular Molecules for Flow Cytometric Analysis in our handbook: Techniques for Immune Function Analysis Application Handbook 1st Edition. 2003 also found at www.bdbiosciences.com.