BD, 554411, BD Pharmingen™ FITC Rat Anti-Mouse IFN-γ

Alternative Name: Ifg; Ifng; IFN-γ; IFN-g; IFN-gamma; Interferon gamma; Type II Interferon
Reactivity: Mouse (QC Testing)
Isotype: Rat IgG1, κ
Immunogen: Mouse IFN-γ Recombinant Protein
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_395375
Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining and Flow Cytometric Analysis: The FITC-conjugated XMG1.2 antibody can be used for multicolor immunofluorescent staining and flow cytometric analyses to identify and enumerate IFN-γ producing cells within mixed cell populations. For optimal immunofluorescent staining with flow cytometric analysis, this anti-cytokine antibody should be titrated (≤0.5 µg mAb/million cells). A useful control for deomonstrating specificity of staining is either of the following: (1) pre-block the conjugated XMG1.2 antibody with a molar excess of ligand (e.g., recombinant mouse IFN-γ; Cat. No. 554587) prior to staining, or (2) pre-block the fixed/permeabilized cells with un-conjugated  XMG1.2 antibody (Cat. No. 554409) prior to staining. The staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcelfe. A suitable rat IgG1 isotype control for assessing the level of background staining on paraformaldehyde-fixed/saponin-permeabilized mouse cells is FITC-R3-34 (Cat. No. 554684); use at comparable concentrations to antibody of interest.