BD, 554503, BD Pharmingen™ Purified Rat Anti-Human GM-CSF

Alternative Name: CSF2; Colony stimulating factor 2 (granulocyte-macrophage); CSF; GMCSF
Reactivity: Human (QC Testing)
Isotype: Rat LEW, also known as Lewis IgG2a
Immunogen: Recombinant human GM-CSF
Application: Intracellular block/flow cytometry (Tested During Development), Immunoprecipitation/Western blot (Reported)
Concentration: 0.5 mg/ml
RRID: AB_398564
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Blocking Control for Intracellular Staining: The purified BVD2-21C11 antibody (Cat. No. 554503) can be used as a blocking control to demonstrate specificity of human GM-CSF staining by the PE-BVD2-21C11 (Cat. No. 554507). To perform this control, the fixed/permeabilized cells (~ 1 million) can be incubated with 1 - 10 µg of unlabeled BVD2-21C11 antibody (Cat. No. 554503) for 20 minutes at 4°C, prior to staining with PE-BVD2-21C11 antibody (eg, 0.1 - 0.5 µg mAb/ 1 million cells). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.  For specific methodology, please visit the protocols section under "Cytokines (Intracellular Staining)" at our website: http://www.bdbiosciences.com/us/s/resources. ELISA Detection: The biotinylated BVD2-21C11 antibody, Cat. No. 554505, is useful as a detection antibody for a sandwich ELISA for measuring human GM-CSF protein levels.  Biotinylated BVD2-21C11 antibody can be paired with the purified BVD2-23B6 antibody (Cat. No. 554502) as the capture antibody, with recombinant human GM-CSF (Cat. No. 550068) as the standard. For specific methodology please visit the protocols sections or the chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com. This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. Note : For testing GM-CSF in serum or plasma, our BD OptEIA™ ELISA Set (Cat. No. 555126) is recommended. IP : The purified BVD2-21C11 antibody has been reported to be useful for immunoprecipitation studies.  Please note that this application is not routinely tested at BD Biosciences Pharmingen.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Ficoll-Paque is a trademark of Amersham Biosciences Limited. Cy is a trademark of GE Healthcare. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.