BD, 554599, BD Pharmingen™ Recombinant Mouse IL-13

Preparation And Storage: Preparation And Storage Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product. Rapidly thaw and quick-spin product prior to use. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, prepare stock solutions diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine serum albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be diluted to less than 5 μg/ml for long term storage. Note: Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.
Recommended Assay Procedures: Recommended Assay Procedures Biological Activity Activity Assay: Measured by proliferation using  TF-1 indicator cells Specific Activity Range :  0.6 - 5 x 10^7 Units*/mg ED50 : 0.2 - 1.5 ng/ml ; Observed dose response relationship: 50 fold * Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation .
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Please refer to http://regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.