BD, 555858, BD Pharmingen™ Biotin Mouse Anti-Human IgE

Alternative Name: Immunoglobulin E; IGHE; IGHε; Ig epsilon chain constant region
Reactivity: Human (QC Testing)
Isotype: Mouse IgG2a, κ
Application: ELISA (Routinely Tested), Flow cytometry (Reported)
Concentration: 0.5 mg/ml
RRID: AB_396180
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures: Recommended Assay Procedures For development of a quantitative 2-site ELISA for measuring IgE using anti-Ig reagents from BD Biosciences Pharmingen or from some other vendor, it is important to empirically determine that there is no epitope blocking/steric hindrance. Please call Techinical Servies for latest information from mAb pairings for these 2-site assays or reference our product information sheet under "ELISA and ELISPOT" at our website: http://www.bdbiosciences.com/us/s/resources.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.