BD, 556029, BD Pharmingen™ PE Mouse Anti- BrdU Set

Reactivity: Human (QC Testing), Mouse, Rat (Tested in Development)
Application: Flow cytometry, Intracellular staining (flow cytometry) (Routinely Tested)
Regulatory Status: RUO
RRID: AB_396305
Description: Description Bromodeoxyuridine (BrdU) is an analog of thymidine that can be incorporated into newly synthesized DNA by cells entering and progressing thru the DNA synthesis (S) phase of the cell cycle.  The amount of BrdU that gets incorporated is dependent upon the amount of time that the cells are exposed to BrdU (pulse time), the rate of cell division, and whether the cells are in early, mid, or late S phase.  Detection of incorporated BrdU allows the investigator to identify cycling cells in an asynchronous cell population and to determine cell cycle kinetics. The 3D4 monoclonal antibody reacts with BrdU, but not other nucleotides, in single-stranded DNA. Random cleavage (nicking) of cellular DNA with DNase I permits the binding of the antibody to incorporated BrdU.
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures Investigators:  Please note that this product is routinely tested on fixed and permeabilized REH cell line with 70-80% cold ethanol. Please see the detailed protocol example at http://www.bdbiosciences.com/us/resources/protocols/s/brdudetection  This product is NOT recommended to use with the BrdU flow kits (e.g. Cat. Nos. 559619, 552598, 557892 and 557892).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application. An isotype control should be used at the same concentration as the antibody of interest.
Description : Quantity/Size : Part Number : EntrezGene ID
N/A : 100.0 : N/A : N/A