BD, 557732, BD Pharmingen™ Alexa Fluor® 647 Mouse IgG1 κ Isotype Control

Isotype: Mouse IgG1, κ
Application: Intracellular staining (flow cytometry), Isotype control (Routinely Tested)
Vol. Per Test: 5 µl
RRID: AB_396840
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Recommended Assay Procedures: Recommended Assay Procedures Intracellular Immunofluorescent Staining Analysis: The MOPC-21 immunoglobulins are suitable mouse IgG1, κ isotype controls for assessing the level of background staining on paraformaldehyde fixed/saponin-permeabilized mouse or human cells for flow cytometric analysis. Use the MOPC-21 antibody at 5 µl test as recommended to serve as an isotype control. BD Pharmingen™ Alexa Fluor® 647 Mouse IgG1 κ Isotype control, Cat. No. 557714 is recommended for non-intracellular staining.
Product Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC). For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.