BD, 559916, BD Pharmingen™ Purified Hamster Anti-Mouse CD120b

Alternative Name: Tnfrsf1b; Tnfr2; TNF-R2; TNFR p75; TNFRII; TNF-R-II; TNFBR
Reactivity: Mouse (QC Testing)
Isotype: Armenian Hamster IgG1, λ3
Immunogen: Mouse Type II TNFR
Application: Flow cytometry (Routinely Tested), Immunoprecipitation (Reported)
Concentration: 0.5 mg/ml
RRID: AB_397371
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining and Flow Cytometric Analysis: The purified form of TR75-89 (Cat. No. 559916) can be used for the immunofluorescent staining (≤ 1 µg antibody/10e6 cells) and flow cytometric analysis of normal mouse cells or cell lines to measure their expressed levels of p75 TNFR. An appropriate purified immunoglobulin isotype control is clone G235-2356 (Cat. No. 553951). A three-layer staining protocol is recommended for maximizing the detection of p75 TNFR expressed by cells as detailed in the figure legend. Note: TR75-89 is a nonblocking antibody that can be used for the unobstructed immunofluorescent staining and flow cytometric analysis of cells in systems where ligands (e.g., TNF) for p75 TNF receptors are present. Based on our testing results (data not shown), the presence of exogenous recombinant mouse TNF (Cat. No. 554589) at levels ≤ 1 µg per 10e6 cells was insufficient to inhibit the binding of TR75-89 to L929 cells that express p75 TNFR (at 0.25 µg antibody/10e6 cells). Please note also that as a consequence of in vivo or in vitro activation, cell surface p75 TNFR can either be shed by cells or transiently expressed at higher levels. As a result, cellular activation can affect the cell's overall expressed level of surface p75 TNFR. IP: The TR75-89 antibody has been reported to be useful for the immunoprecipitation of p75 TNFR from lysates of mouse Meth A fibrosarcoma cells. Please note that this application is not routinely tested at BD Biosciences Pharmingen.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.