BD, 561845, BD Pharmingen™ FITC Rat Anti-Mouse CD40

Reactivity: Mouse (QC Testing)
Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Immunogen: Mouse CD40 Recombinant Protein
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_10897019
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures Flow Cytometry: The staining intensity of FITC-conjugated 3/23 mAb is severely reduced when used on leukocytes that have been fixed (≤ 2.5 hours with ~0.5% formaldehyde). Therefore, investigators are advised not to fix cells prior to staining for flow cytometry. Freshly-isolated leukocytes and cell lines may wait for analysis in wash buffer at 4°C, without fixation, for up to 18 hours post-staining without loss of viability. Activated lymphocytes may lose viability rapidly, and data should be collected within 5 hours post-staining. For applications requiring fixation of cells prior to staining, an alternate mAb to mouse CD40, HM40-3 (Cat. No. 553723), is recommended.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.