BD Horizon™ BV480 Rat Anti-Mouse CD45R/B220 565631

Alternative Name: B220; Ly-5; CD45R; LCA; Ptprc; Protein tyrosine phosphatase receptor type C
Reactivity: Mouse (QC Testing), Human (Tested in Development)
Isotype: Rat IgG2a, κ
Immunogen: Mouse Abelson Leukemia Virus-Induced pre-B tumor cells
Application: Flow cytometry (Routinely Tested), Immunofluorescence (Tested During Development)
Concentration: 0.2 mg/ml
RRID: AB_2739311
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV480 under optimum conditions, and unconjugated antibody and free BD Horizon BV480 were removed.
Recommended Assay Procedures: Recommended Assay Procedures For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349). For Immunofluorescence Applications: The use of a mounting reagent (eg, ProLong® Gold) is highly recommended to maximize the photostability of BV480.  For confocal microscopy systems, a 440 nm laser is the optimal excitation source and the recommended emission filter is a 485/20 nm bandpass filter. For epifluorescence microscopes with broad spectrum excitation sources,  the recommended excitation and emission filters are 445/20 nm and 485/20 nm bandpass filters, respectively.  For specific multicolor imaging applications, the exact filter configurations should be optimized by the end user. For additional instrument/filter configuration information, please visit http://www.bdbiosciences.com/research/cellularimaging.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. This antibody has been developed for the immunofluorescence imaging application. However, the antibody is routinely QC tested by flow cytometric analysis. Researchers are encouraged to titrate the reagent for optimal performance. BD Horizon Brilliant Violet 480 is covered by one or more of the following US patents: 8,575,303; 8,354,239. ProLong® is a registered trademark of Thermo Fisher Scientific, Inc. Waltham, MA. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.