BD Horizon™ BV480 Mouse Anti-Human CD45RO 566143

Alternative Name: CD45R; PTPRC; LCA; Leukocyte common antigen; GP180; LY5; T200
Reactivity: Human (QC Testing)
Isotype: Mouse BALB/c IgG2a, κ
Immunogen: Human IL-2-dependent T-cell line
Application: Flow cytometry (Routinely Tested)
Vol. Per Test: 5 µl
Workshop Number: IV N31
RRID: AB_2739541
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The antibody was conjugated with BD Horizon BV480 under optimum conditions, and unconjugated antibody and free BD Horizon BV480 were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices: Product Notices BD Horizon Brilliant Violet 480 is covered by one or more of the following US patents: 8,575,303; 8,354,239. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Source of all serum proteins is from USDA inspected abattoirs located in the United States. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest.