Qiagen, 290133, mericon Vibrio triple Kit (24)

For 24 reactions: PCR Assay Vibrio triple, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Features

- Highly sensitive and specific detection of a broad range of pathogens
- Single, streamlined detection protocol for all targets
- Dedicated mericon bacterial DNA isolation kits available
- PCR run time of 73 minutes on the QIAGEN Rotor-Gene Q
- Full license for PCR without additional costs

Product Details

mericon Pathogen Detection Assays are a suite of kits for the target-specific detection of bacterial pathogen DNA in food, animal feed, and pharmaceutical products. All of the assays use the same real-time PCR-based protocol. They are part of the comprehensive QIAGEN food safety testing portfolio, which also features dedicated sample preparation kits and assays for ingredient authentication. mericon assays perform optimally on the Rotor-Gene Q, but have also been validated for block thermal cyclers.

Performance

Pathogen: Result
Campylobacter jejuni: +
Campylobacter lari: +
Salmonella abony: –
Clostridium perfringens: –
Legionella erythra: –
Legionella pneumophila: –
Bacillus cereus: –
Shigella flexneri: –

Pathogen: Result
Cronobacter sakazakii: +
Cronobacter turingensis: +
Cronobacter muytjensii: +
Campylobacter jejuni: –
Campylobacter coli: –
Campylobacter lari: –
Campylobacter upsaliensis: –
Staphylococcus aureus: –
Bacillus cereus: –
Legionella erythra: –

Pathogen: Result
Legionella pneumophila: +
Legionella adelaidensis: +
Legionella impletisoli: +
Legionella israeliensis: +
Legionella worsleinsis: +
Legionella spiritensis: +
Legionella brunensis: +
Legionella geestiana: +
Legionella santicruci: +
Salmonella abony: –
Clostridium perfringens: –
Escherichia coli: –
Staphylococcus aureus: –
Shigella flexneri: –

Pathogen: Result
Vibrio vulnificus: +
Vibrio parahaemolyticus: +
Vibrio cholerae: +
Vibrio alginolyticus: –
Vibrio furnissii: –
Legionella pneumophila: –
Campylobacter jejuni: –
Listeria monocytogenes: –

mericon Campylobacter spp Kit

The mericon Campylobacter spp Kit is for the specific detection of several Campylobacter subspecies in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Campylobacter DNA in a reaction and it exhibits high specificity for pathogens this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Campylobacter spp Kit cross-reactivity study").

mericon Cronobacter spp Kit

The mericon Cronobacter spp Kit is for the specific detection of Chronobacter subspecies in enrichment cultures of food and animal feed samples. Due to a recent change in taxonomy and according to regulation of the EU Commission (365/2010) the name Cronobacter spp replaces Enterobacter sakazakii . The assay can detect as few as 10 copies of Cronobacter DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Cronobacter spp Kit cross-reactivity study").

mericon Quant Legionella spp Kit

The mericon Quant Legionella spp Kit is for the specific detection and quantification of several Legionella species in water after concentration (e.g., by filtration). The assay can detect as few as 10 copies of Legionella DNA in a reaction and it exhibits high specificity for pathogens of that genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Quant Legionella spp Kit cross-reactivity study").

mericon Salmonella spp Kit

The mericon Salmonella spp Kit is for the specific detection of several Salmonella subspecies in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Salmonella DNA in a reaction and it exhibits high specificity for pathogens of this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens.

mericon Vibrio triple Kit

The mericon Vibrio triple Kit is for the specific detection of Vibrio vulnificus , Vibrio parahaemolyticus , and Vibrio cholerae in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Vibrio vulnificus , Vibrio parahaemolyticus , and Vibrio cholerae DNA in a reaction and exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Vibrio triple Kit cross-reactivity study").

mericon E. coli O157 Detection Kits

The mericon E. coli Screen Plus Kit is designed for the qualitative detection of the E. coli serotype O157 and E. coli virulence genes intimin (eae) and Shiga toxin-like proteins (stx1 and stx2). The presence of eae and stx1/stx2 suggests that further investigation into the E. coli O-serotype O157, or the non-O157 O-serotypes (O26, O45, O103, O111, O121, and O145) is required in food and animal feed samples after enrichment. The assay can detect as few as 10 copies of E. coli DNA in a reaction and it exhibits high specificity for pathogens of this species. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see table "Results of mericon E. coli O157 Screen Plus Kit cross-reactivity study" for exclusivity/inclusivity data).

The mericon E. coli STEC O-Type Kit exhibits high specificity for E. coli strain O157:H7, O26, O45, O103, O111, O121, and O145. The kit was tested against an extensive inclusivity panel of E. coli O157:H7 and non-O157:H7 serotype strains, and an exclusivity panel of other E. coli and non- E. coli strains. All tested O157:H7 and non-O157:H7 serotype strains (O26, O45, O103, O111, O121, and O145) were successfully detected and no cross-reactivity was observed with other pathogens using 2500 copies of tested DNA.

Principle

Internal control amplified: Tested DNA amplified
+: +
+: –
–: –

Detection of pathogens in food testing using real-time PCR is based on the amplification of a specific region of the relevant pathogen genome. The amplified product is detected using target-specific fluorescent probes that bind to it. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes afterward.

mericon PCR Assays include an internal control to control potential inhibition. This aids in the straightforward interpretation of the results (see the table "Summary of possible PCR outcomes").

The PCR primer set for each assay is highly specific and targets a unique and conserved region of the tested pathogen genome that has been bioinformatically and experimentally verified.

Procedure

DNA is extracted from an enrichment culture of a sample of food or animal feed using a dedicated sample preparation kit. The mericon Bacteria Kit is for the isolation of bacterial DNA from Gram-negative bacteria. The mericon Bacteria Plus Kit is for the isolation of bacterial DNA from Gram-positive bacteria.

The isolated, pure bacterial DNA is assayed using a streamlined real-time PCR protocol that returns results in 73 minutes on QIAGEN's Rotor-Gene Q. The protocol has also been validated for use on other thermal block cyclers.

The results are displayed as easy-to-interpret PCR curves.

Applications

mericon Pathogen Detection Assays are for real-time PCR-based detection of specific bacterial pathogens in food, animal feed and pharmaceutical products.