Qiagen, 339160, miRCURY LNA miRNA Power Family Inhibitors

Predesigned sets of miRCURY LNA miRNA Inhibitors for miRNA families

Features

- Enable functional analysis of an entire miRNA family, instead of just individual family members
- LNA design ensures that all miRNA family members are silenced with similar high efficacy, regardless of GC content
- More than 40 predesigned inhibitors of miRNA families available
- Allows discovery of novel miRNA functions that would not be revealed when analyzing individual family members

Product Details

miRCURY LNA miRNA Family Power Inhibitors allow you to study regulatory roles shared by highly related, co-expressed and functionally redundant miRNAs. Such functions would not be revealed in analyses using inhibitors of individual family members. These antisense oligonucleotides have perfect sequence complementarity to their targets, and when introduced into cells, they sequester the target miRNA in highly stable heteroduplexes that prevent interaction with the normal cellular partners.

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Performance

Discover unknown miRNA function

Principle

- Sequence optimization: identification of a target sequence sufficiently short to be shared by family members and sufficiently long to define the family.
- Mixed-base synthesis: when target sequences differ at a single position, we use a mixture of two bases at the corresponding position during the synthesis of the antisense oligonucleotide. This allows us to generate two oligonucleotides in a single oligonucleotide synthesis reaction.
- Mixture of inhibitors: when it is not possible to target a whole family using a single nucleotide synthesis, we provide a mixture of two or more inhibitors that make up the family inhibitor.
- LNA optimization: family inhibitors are short 10–16mers, but enhanced LNA content ensures that they all have the same target affinity (T m ) as our individual miRCURY LNA miRNA inhibitors.

Design

Coverage