Qiagen, 339200, miRCURY LNA miRNA Power Target Site Blockers, in vivo Ready (15 nmol)

15 nmol miRCURY LNA miRNA Power Target Site Blockers, in vivo Ready, with option of different labels and purifications, provided in tube format

Features

- Custom-designed target site blockers for specific inhibition of miRNA targets
- Sophisticated design and superior high affinity, regardless of target sequence
- Unmatched high efficacy in vitro and in vivo
- Unrivaled performance and high protein expression due to lack of RNase H-dependent mRNA degradation
- Efficient at very low concentrations, outcompeting miRNAs for their target sites
- Superior biological stability for long-lasting antisense activity

Product Details

miRCURY LNA miRNA Power Target Site Blockers are antisense oligonucleotides that bind to the miRNA target site of an mRNA, preventing miRNAs from gaining access to that site. This enables you to study the effects of an miRNA on a single target. miRCURY LNA miRNA Power Target Site Blockers are efficient at very low concentrations, and due to their high affinity from LNA enhancement , the target site blockers outcompete miRNAs for their target sites.

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Performance

- Determine which pathway is involved in a phenotype observed upon miRNA inhibition
- Determine which miRNA/mRNA interactions are most important in a pathway containing several predicted targets

Unravel miRNA function with target site blockers

Principle

What are target site blockers?

miRCURY LNA miRNA Target Site Blockers are LNA-enhanced antisense oligonucleotides that bind to the miRNA target site of an mRNA, thereby preventing miRNAs from gaining access to that site. This allows researchers to study the effects of an miRNA on a single target. In contrast, the phenotype observed when inhibiting an miRNA reflects the combined effects of that miRNA on all targets.

LNA-enhanced target site blockers

The incorporation of LNA into the miRCURY LNA miRNA Power Target Site Blocker means that they will compete more effectively with the miRNA/RISC complex for the miRNA target site. In addition, LNA distribution throughout the LNA/DNA mixmer ensures that the antisense oligonucleotide does not catalyze RNase H-dependent degradation of the mRNA. As a result, the TSB will cause increased expression of the protein encoded by the targeted mRNA by preventing miRNA-mediated translational attenuation (see figure LNA-enhanced target site blockers compete effectively with RISC for miRNA binding site ).

Applications

- Determining which pathway is involved in a phenotype observed upon miRNA inhibition
- Determining which miRNA/mRNA interactions are most important in a pathway containing several predicted targets