Qiagen, 673613, JAK2 RGQ PCR Kit (24) RUO

For 24 reactions: V617F Positive Control, V617F Negative Control, 2 sets of standards plasmid dilutions JAK2 wild-type and JAK2 V617F, qPCR master-mix, 2 Primers and Probe Mixes JAK2 wild-type and JAK2 V617F including internal control

Features

- Reliable and sensitive quantification of JAK2 V617F
- Larsen’s primers in line with most recent international technical recommendations (1)
- Internal control included
- All-in-one, ready to use solutions including QIAGEN master mix
- Easy-to-use protocols validated on the QIAsymphony SP and the Rotor-Gene Q 5plex HRM
- Optimized turn-around time

Product Details

The JAK2 RGQ PCR is intended for real-time PCR on the Rotor-Gene Q 5plex HRM. The kit provides all the reagents optimized for reliable and sensitive detection, and quantification of the JAK2 V617F/G1849T mutation in genomic DNA.

Performance

To ensure improved sensitivity and reproducibility, the JAK2 RGQ PCR Kit has been optimized to detect the JAK2 V617F/G1849T mutation and uses prediluted plasmid standards, primers, and probe mixes in line with recent international technical recommendations (1). The limit of detection (LOD) is 0.042%.

Principle

The JAK2 RGQ PCR Kit is a ready-to-use kit for the detection and quantification of the JAK2 V617F/G1849T somatic mutation due to a single nucleotide polymorphism (SNP) in genomic DNA. The kit use polymerase chain reaction (PCR) and amplification refractory mutation system (ARMS) technology on Rotor-Gene Q 5plex HRM.

Procedure

The JAK2 RGQ PCR Kit allows detection and quantification of the JAK2 V617F/G1849T mutation on Rotor-Gene Q 5plex HRM. The first step is manual or QIAsymphony-automated DNA extraction and determination of quality and quantity of genomic DNA. The second step is the amplification of the genomic DNA by real-time PCR, and all the reagents needed for this step are provided in the kit. Simply start the reaction on the Rotor-Gene Q 5plex HRM using the optimized protocols as described in the kit handbook.

Applications

The JAK2 RGQ PCR Kit enables sensitive and reliable detection and quantification of the JAK2 V617F/G1849T mutation. This kit is for research use only.