Qiagen, 9003110, QIAxcel Connect

Capillary electrophoresis device: includes computer, QIAxcel ScreenGel Software, and 1-year warranty on parts and labor

Features

- Ready-to-use gel cartridges
- High sensitivity, high resolution
- Process up to 96 samples without manual intervention

Product Details

- High-resolution genotyping
- Fast single or multiplex PCR screening
- QC of cell-free DNA
- NGS library QC
- Fast single-amplicon analysis, such as HLA typing
- RNA quality checks

Fully automate the fragment analysis and quality control of up to 96 samples per run with QIAxcel Connect. This capillary-electrophoresis instrument uses ready-to-run gel cartridges that eliminate tedious gel or consumable preparations. It also reduces handling errors.

Various QIAxcel Kits meet your specific needs (see High versatility with multiple kits ):

And if you want to monitor your runs from outside the lab, you can connect to the optional QIAsphere system on your instrument and phone.

Principle

QIAxcel Connect uses prefilled gel cartridges to perform automated capillary electrophoresis. Simply place samples and buffers into the instrument and start the run. The tips of the gel cartridges move into the sample wells. An electric field creates a positive charge. This makes the negatively charged nucleic acid fragments migrate through the gel in the capillaries, with the smaller fragments travelling faster than the larger ones.

Within the capillary, a dye intercalates into the nucleic acid. As the fragments pass a detector, it measures the signals emitted by the dye. The QIAxcel ScreenGel software converts these signals to digital data; you can see them in real time as electropherograms or gel images (see Cartridge-based gel electrophoresis ).

Procedure

Turn on the instrument and put in the buffer tray filled with buffer and markers. Insert the QIAxcel Gel Cartridge and your samples into the instrument. Select your analysis protocol and run the samples. See your results in real time, displayed on your QIAxcel ScreenGel user interface (see Plug-and-play electrophoresis ).

Applications

- Next-generation sequencing (NGS) libraries
- Low-concentration samples, such as liquid biopsy
- Degraded samples, such as formalin-fixed paraffin-embedded (FFPE) tissue
- Multiplex PCR products with high-resolution requirements

QIAxcel Connect is highly suitable for analysis and quality control of: