Thermo Fisher, 11319019, ElectroMAX™ DH5α-E Competent Cells

ElectroMAX™ DH5α-E Competent Cells ElectroMAX DH5α-E Competent Cells are derived from the DH5α strain and are suitable for transformation by electroporation. It is challenging to achieve high transformation efficiency by electroporation using the standard DH5α strain (chemical transformation is more efficient) thus additional mutations were introduced to create the DH5α-E strain that is able to reach >1 x 1010efficiency by electroporation with non-saturating amounts of DNA. Using this strain, 10 to 50 times greater colony number may be obtained in a single transformation than using the chemically competent DH5α cells. Therefore DH5α-E competent cells are ideal for challenging applications, such as generation of cDNA libraries or in transformations with limited DNA input.
ElectroMAX DH5α-E Competent Cells share several key genetic properties. They carry thehsdR mutation to prevent restriction of foreign DNA and theendA1 mutation that increases the yield of plasmid DNA prepared using miniprep protocols. ThelacZΔM15 marker provides α-complementation of the ß-galactosidase gene, allowing blue-white screening on agar plates containing X-gal or Bluo-gal. DH5α-E allows efficient transformation of large plasmids and can also serve as a host for M13mp cloning vectors if a lawn of DH5αF′IQ is provided to allow plaque formation. We do not recommended using this strain for direct cloning of methylated genomic DNA.
ElectroMAX DH5α-E Competent Cell features
• Greatly increased plasmid yield and quality due toendA1 mutation
• High-efficiency transformation with plasmids 30 kb in size
• Blue/white screening of recombinant clones due tolacZΔM15
• Ensured insert stability due torecA1 mutation
•hsdRfor efficient transformation of unmethylated DNA from PCR applicationsNote:A high-voltage electroporation apparatus is required.
GenotypeF–φ80lacZΔM15 Δ(lacZYA-argF)U169recA1endA1hsdR17(rK–, mK+)galphoAsupE44 λ-thi-1gyrA96relA1Genetic marker descriptionsFind the strain and format that fits your needsDH strains are available inchemically competentandelectrocompetent cellformats.
ExploreMultishot formatfor high throughput applications.
MAX Efficiency DH5αF´IQ Competent Cellsare available in chemically competent format.
Explorebacterial growthmedia formats.
Antibiotic Resistance Bacterial: No
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: No
Cloning Unstable DNA: Not suitable for cloning unstable DNA
Contains F' Episome: No
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Plasmid: May be used for plasmids >20 kb
Preparing Unmethylated DNA: No
Product Line: ElectroMAX™
Product Type: Electrocompetent Cells
Quantity: 5 x 100 μL
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Transformation Efficiency Level: High Efficiency (>1 x 109cfu/μg)
Format: Tube
Species: E. coli (K12)
Unit Size: Each