Thermo Fisher, 23030, Pierce™ NEM (N-ethylmaleimide)

Thermo Fisher, 23030, Pierce™ NEM (N-ethylmaleimide) Thermo Scientific Pierce N-Ethylmaleimide (NEM) is a small compound that forms stable, covalent thioether bonds with sulfhydryls (e.
g., reduced cysteines), enabling them to be permanently blocked to prevent disulfide bond formation.
Features of N-Ethylmaleimide (NEM):
• Permanently block sulfhydryls to prevent disulfide bond formation.
• Monitor the reaction by measuring the decrease in absorbance at 300nm
• Block sulfhydryl-containing reagents that interfere in enzyme assaysN-Ethylmaleimide (NEM) is an alkylating reagent that reacts with sulfhydryls. At pH 6.
5-7.
5, the maleimide reaction is specific for sulfhdryls; however, at pH values greater than 7.
5, reactivity with amino groups occurs. Maleimide groups react with sulfhydryls by nucleophilic attack of the thiolate anion on one of the carbons of the double bond. When sufficient sulfhydryls have been blocked, the reaction can be monitored by measuring the decrease in absorbance at 300nm as the double bond becomes a single bond. The resulting thioether group is non-reversible and terminates in an ethyl group, blocking or capping the sulfhydryl.
Form: Solid
Label Type: Blocking Groups
Label or Dye: Unreactive Group
Product Line: Pierce™
Quantity: 25 g
Reactive Moiety: Maleimide
Solubility: Water
Chemical Reactivity: Thiol
Product Type: NEM
Spacer: Medium (10 to 30 Å)
Unit Size: Each