Thermo Fisher, 4471269, Ion Xpress™ Plus Fragment Library Kit

Ion Xpress™ Plus Fragment Library Kit The Ion Xpress™ Plus Fragment Library Kit provides a rapid and flexible enzymatic-based library construction methodology for the Personal Genome Machine™ sequencing system workflow. Employing enhanced proprietary Ion Shear™ DNA fragmentation chemistry, this revolutionary kit allows completion of library preparation in as little as 2 hours for gDNA and amplicon libraries, removing the need for a physical shearing device to save time and cost. With significantly higher yields and lower bias than other library construction techniques, superior coverage uniformity is attained with as little as 100 ng input DNA. In addition, highly efficient library construction enables the creation of 'amplification-free' libraries from 1 μg input material. This kit allows users to modulate an appropriate fragment insert size depending on desired read length and application. The Ion Xpress™ Plus Fragment Library Kit contains sample preparation reagents for enzymatic shearing and library construction for up to 20 DNA libraries (depending on input DNA type) for semiconductor sequencing. Store components at -20°C. The Ion Xpress™ Plus Fragment Library Kit offers you:Superior coverage uniformity for diverse sample types using our proprietary Ion Shear™ Plus chemistryLower input requirement with as little as 100 ng starting materialRapid and flexible workflow with Ion fragment libraries containing variable insert sizes in as fast as 2 hoursSignificant cost savings by removing the need for ancillary shearing devicesScalable methodology applicable to automated library preparation systems including the AB Library Builder™ SystemA Single-Day Workflow: The Next Stage in a Sequencing RevolutionWith a turnaround time of as little as 2 hours, the Ion Xpress™ Fragment Library Kit shortens the Ion sequencing workflow to a single workday, making comprehensive analyses of your sample(s) of interest possible in less time than ever before. Furthermore, Ion Xpress™ Plus fragment libraries exhibit greater coverage uniformity and higher recovery rates across diverse sample types than other library construction methodologies, so you can be confident of generating the best data in the shortest time. Ion Torrent Sequencing Made Simpler, Faster, and More AffordableIon Shear™ Plus DNA fragmentation technology, included in the Ion Xpress™ Plus kit, obviates the need for costly physical shearing devices for most applications and requires as little as 100 ng of sample, accelerating your path to semiconductor sequencing. This flexible technology provides users the ability to adjust the resulting DNA fragment size to accommodate a range of sequencing read lengths depending on their project requirements. This technology is also amenable to automated library preparation systems such as the AB Library Builder™ System to provide quick answers when you need them most. Ion Shear™ Plus DNA fragmentation technology is not included in the Ion Plus kit. Use the Ion Plus kit if physical shearing methods are preferred. For Use With (Application): Sequencing
For Use With (Equipment): Ion GeneStudio™ S5 Series Sequencer, Ion Chef™ System
High-throughput Compatibility: High-throughput Compatible, Multiplexing
Libraries: Fragment Library
No. of Reactions: 10
Product Line: Ion Torrent™, Ion Xpress™
Product Type: Plus Fragment Library Kit
Quantity: 10 Reactions
Sample Type: DNA
Technique: Post-Light™ Ion Semiconductor Sequencing
Template Compatibility: Genomic DNA
Workflow Step: Library Generation
Format: Kit
Sequencing Type: Genome and DNA Sequencing
Unit Size: Each