Thermo Fisher, ER1981, SmoI (SmlI) (10 U/μL)

Thermo Fisher, ER1981, SmoI (SmlI) (10 U/μL) 5'C ↓TYRAG3'3'GARYT ↑C5'Thermo Scientific SmoI (SmlI) restriction enzyme recognizes C^TYRAG sites and cuts best at 55°C in Tango buffer. SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Isoschizomers: SmlI.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificitiesApplications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNPNote:Incubation at 37°C results in 10% activity. SmoI cleavage is impaired by overlapping dam methylation. To avoid dam methylation, use a dam-, dcm- strain such as GM2163.
Low salt, high glycerol (>5%) concentrations, pH > 8.
0 or a large excess of enzyme may result in star activity. For methylation sensitivity, refer to product specifications.
Compatible Buffer: 10X Buffer Tango
Product Type: Restriction Enzyme
Quantity: 200 units
Concentration: 10 U/μL
Enzyme: SmoI (SmlI)
Methylation Sensitivity: Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Optimal Reaction Temperature: 55°C
Research Category: Traditional Cloning
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Unit Size: Each