Thermo Fisher, K1691, RevertAid RT Reverse Transcription Kit

RevertAid RT Reverse Transcription Kit The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), which has lower RNase H activity compared to AMV reverse transcriptase. The enzyme maintains activity at 42–50°C and is suitable for synthesis of cDNA up to 13 kb. The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55°C.
First strand cDNA synthesized with this system can be directly used as a template in PCR or real-time PCR. It is also ideal as a first step for second strand cDNA synthesis or linear RNA amplification. Radioactively and non-radioactively labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments, including microarrays.
Features of the RevertAid RT Kit include:
• Full-length first strand cDNA up to 13 kb
• Optimum reaction temperature 42°C
• Complete kit includes all the components for RT reactionsApplications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesisFinal Product Type: First-Strand cDNA
Format: Kit
No. of Reactions: 500 Reactions
Optimal Reaction Temperature: 42°C
Quantity: 500 reactions
Reaction Format: Separate components
Reagent Type: Reverse Transcription
Reverse Transcriptase: RevertAid
Ribonuclease H Activity: Yes
Shipping Condition: Dry Ice
Size (Final Product): Up to 13 kb
Starting Material: RNA
Technique: Reverse Transcription
For Use With (Application): Real Time PCR (qPCR)
Reaction Speed: 60 min.
Unit Size: Each