Waters, 186008960, BioResolve RP mAb Polyphenyl Method Validation Kit, 450Å, 2.7 µm, 4.6 mm X 100 mm, 3/pk

The first choice for reverse phase protein separation and analysis, BioResolve RP mAb Polyphenyl Method Validation Kit, 450Å, 2.7 µm, 4.6 mm X 100 mm Columns contain 2.7 µm superficially-porous-silica particles having an average pore diameter of 450Å. The particles are functionalized with a proprietary polyphenyl bonding process that was specifically designed for LC or LC-MS analyses of intact IgG monoclonal antibodies and their fragments. Each batch is qualified using a gradient separation of the Waters mAb Subunit Standard (p/n: 186008927). This Method Validation Kit (MVK) Part Number contains (3) (p/n: 186008954) columns from (3) different manufactured batches. Note: p/n: 176004170 includes the (3), 4.6 x 100mm (p/n: 186008954) columns from (3) different manufactured batches plus three vials of the Intact mAb Mass Check Standard (p/n: 186006552) and three vials of the mAb Subunit Standard (p/n: 186008927). Suggested Use: Intact protein and mAb subunit analysis, Protein Analysis, ADC characterization, AAV, Antibody Conjugates

Specifications
Separation Mode: Reversed Phase
Temperature Limits: 90 C
Maximum Pressure: 10000 psi (689 Bar)
Particle Size: 2.7 µm
Pore Size: 450 Å
QC Tested: Protein
System: HPLC, UHPLC, UPLC
USP Classification: L11
UNSPSC: 41115709
Brand: BioResolve
Product Type: Column Kits
Units per Package: 3 pk

Additional Information
BioResolve RP mAb Polyphenyl Method Validation Kit, 450Å, 2.7 µm, 4.6 mm X 150 mm, 3/pk Validating methods during monoclonal antibody and antibody drug conjugate analysis can sometimes be difficult, as the need to constantly reproduce under demanding chromatographic conditions can present some analytical challenges to overcome. But with the BioResolve RP mAb Polyphenyl Method Validation Kit, you need not worry about those challenges. This breakthrough mAb and ADC analysis kit includes three BioResolve RP mAb Polyphenyl Columns for your separations and three vials each of the Intact mAb Mass Check Standard and mAb Subunit Standard for use on LC-MS or MS systems and instruments for performance verification purposes. What’s more, these three columns included offer the same sorbent from three different batches, so you can easily put your trust into the reproducibility provided by this solution kit. Take your analyses to the next level when you buy the BioResolve RP mAb Polyphenyl Method Validation Kit. As you work hard to validate your method, you’ll be met with challenges that could cause your column to foul if not solved properly. Column contamination should always be at the front of your mind when in the lab and developing separations, which is why we’re happy to provide a reusable column protection solution with the VanGuard column protection system. Developed out of the growing need for columns to last for longer and perform faster, VanGuard provides the protection your analytical column needs from contaminants that may be lingering in the mobile phase stream. Plus, when the VanGuard cartridge becomes worn and needs to be replaced, you can do so with another genuine replacement from Waters. Add the BioResolve RP mAb Polyphenyl VanGuard Cartridge to your workflow for enhanced column protection. When you purchase the BioResolve RP mAb Polyphenyl Method Validation Kit, you’re receiving three mAb separation columns that offer superficially porous silica particles that are functionalized by a novel polyphenyl bonding process. This process enables this column to provide the rigidity and flexibility needed for the reversed-phase analysis of monoclonal antibodies and their fragments. But even more, the BioResolve RP mAb Polyphenyl Column can be used on all UHPLC, UPLC and HPLC systems despite changing chromatographic conditions across those systems. This flexibility speaks to the rigidity exhibited by this column. Ready to perform successful method development? Before checking out, shop lab equipment to explore all of the other incredible products and resources available at Waters.

FAQ
How Can I Achieve Column Equilibrium with the BioResolve RP mAb Polyphenyl Column? To achieve column equilibrium with the BioResolve RP mAb Polyphenyl Column, flush the column with ten column volumes of a water/organic solvent mixture, using the same or lower solvent content as in the desired buffered mobile phase.