Thermo Fisher Filters & Filtration for Cell Culture and HPLC

Thermo Fisher’s Filters & Filtration range covers sterile bottle-top units, syringe filters, and membrane discs used for clarifying, sterilizing, and protecting analytical instruments. This collection page helps you quickly select by application, membrane, and pore size. As a distributor, Iright supplies a broad mix of Thermo Fisher options with technical support and quoting.

Quick Selection Guide: Match Application to Filter Type & Pore Size

Choosing filters is easier when you map the task to a membrane and pore size first, then pick the right format. Use the grid below to jump-start selection; you can fine-tune by solvent, sample load, and downstream workflow on the product pages that follow.

Use-Case → Recommended setup (fast pick)

Tip: use 0.22 µm when sterility or microbial retention is required; adopt 0.45 µm for general clarification to maximize speed. Verify solvent compatibility when switching between aqueous and organic phases.

Popular Sub-categories: Nalgene Rapid-Flow, Syringe Filters, Membrane Discs

Once you know the target pore size and membrane, choose a format that fits volume and throughput. Start with these high-use families and compare a few representative SKUs to get the feel for sizing and materials.

A) Nalgene™ Rapid-Flow™ Sterile Bottle-Top Filters

Designed for speed and high throughput, Rapid-Flow units are available with PES, CN, SFCA, or Nylon membranes and in common capacities from 150 to 1000 mL. They’re widely used for sterilizing media and buffers and ship sterile for immediate lab use.

Representative SKUs (verify against your volume needs):

• 290-4545 — Bottle-top, sterile; Rapid-Flow series (PES/SFCA/CN/Nylon options shown on page).

• 296-4545 — Bottle-top, sterile; Rapid-Flow series (catalog variant). 

• 295-3345 — Bottle-top, sterile; FAQ notes membranes are molded-in and cannot be removed.

B) Syringe Filters for Sample Prep (Titan3™, Target2™, Choice™)

Syringe filters protect chromatography columns and detectors from particulates. Pick a diameter (13/17/25/30 mm) to match sample load and backpressure; then choose a membrane for compatibility and recovery. Thermo Scientific offers robust lines for routine and demanding samples. 

Representative SKUs (by series/membrane):

• Titan3™ Nylon — 44504-NN (0.45 µm, 4 mm); 44513-NN (0.45 µm, 17 mm); 42225-NN (0.2 µm, 30 mm, prefilter).

• Titan3™ PTFE (Hydrophilic) — 44513-NPL (0.45 µm, 17 mm); 42225-NPL (0.2 µm, 30 mm). Hydrophilic PTFE supports polar solvents without wetting agents.

• Target2™ Nylon — F2500-2 (0.25 µm, 30 mm); F2504-2 (0.2 µm, 4 mm). Cost-effective for routine aqueous/organic blends. 

• Target2™ PTFE — F2500-13 (1.0 µm, 30 mm) with family options at 0.45/0.2 µm and 4/17/30 mm diameters on the same page.

• Choice™ Nylon/PTFE — CH2213-NN (Nylon 0.2 µm, 13 mm); CH2213-NPL (Hydrophilic PTFE 0.2 µm, 13 mm). Budget-friendly, widely used in HPLC prep.

C) Membrane & Prefilter Discs

For vacuum manifolds or reusable holders, discs in standard diameters (e.g., 47 or 50 mm) cover routine clarification and higher loads. Choose CA/PES for aqueous streams; glass-fiber prefilters help with turbid samples.

Representative SKUs:

• DS0210-4045 — CA membrane disc, 47 mm, 0.45 µm (case of 100).

• DS0281-5000 — Glass-fiber prefilter disc, 50 mm (case of 100).

How to Choose Membrane & Micron Size (PES, PVDF, PTFE, Nylon)

Your membrane determines recovery and compatibility; pore size sets retention and speed. Start from sample chemistry, then check protein binding, extractables, and solvent resistance. Use the notes below to avoid common pitfalls in recovery or flow.

• 0.2/0.22 µm vs 0.45 µm: Select 0.2–0.22 µm for sterility/microbial removal; choose 0.45 µm for non-sterile clarification and faster filtration. For chromatography, 0.22 or 0.45 µm both protect the column—pick based on viscosity and sensitivity.

• PES: Low protein binding and low extractables—excellent for serum/media sterilization and general aqueous filtration. Rapid-Flow PES is a common default in cell culture labs.

• Nylon: Tough, broadly compatible with mixed aqueous/organic samples; a routine choice for HPLC prep (Target2, Titan3, Choice families). 

• PVDF: Available in hydrophilic/hydrophobic forms; good chemical resistance with moderate protein binding—useful across aqueous and organic systems (see series pages for specific variants).

• PTFE: The go-to for strong organic solvents and aggressive chemicals. Hydrophilic PTFE variants remove the need for pre-wetting with alcohol for aqueous samples.

Practical rule: if protein recovery matters (e.g., conditioned media, lysates), prioritize PES/SFCA. For solvent-rich samples or method development in GC/LC, PTFE is safest; swap to Nylon or PVDF when you need higher flow with mixed phases. Cross-check the exact membrane description on the SKU page you select.

Compatibility & Setups: Bottle-Top Units, Vacuum Manifolds, HPLC Prep

Compatibility is mostly about interfaces (neck sizes, syringe diameters) and the support equipment you already have. Confirm your receiving bottle/holder dimensions, vacuum source, and instrument requirements to keep prep smooth and reproducible.

• Bottle-top units: Rapid-Flow tops are sold sterile and pair with matching receiving bottles or standard GL necks; they are designed for fast flow and high throughput. The membranes are molded in (not user-removable).

• Syringe filters: Choose 13 mm for small volumes and narrow syringes; 17–25–30 mm for higher loads or viscous samples. Pre-filters (glass fiber) on some models keep the flow steady with particulate-rich samples.

• Disc filters + vacuum: Standard 47/50 mm discs fit common vacuum holders/manifolds and are ideal for batch clarifications; pair a membrane with a GF prefilter for turbid matrices.

FAQs: 0.22 vs 0.45 µm, Sterility, Solvent Compatibility

We collected frequent pre-purchase questions from Thermo Fisher product pages so you can check the essentials in one place before you order. If you still have edge cases, Iright can help confirm membrane/solvent charts.

Q1: Do I need 0.22 µm or 0.45 µm?
Use 0.22 µm for microbial retention or sterile media; use 0.45 µm for quick clarification when sterility is not required (typical HPLC prep).

Q2: Which membrane minimizes protein loss?
PES and SFCA generally show low protein binding, making them suitable for media and serum filtration. 

Q3: Are Rapid-Flow bottle-top membranes removable for cleaning?
No. They’re molded in and not user-removable. 

Q4: What if my sample contains strong organic solvents?
Select PTFE syringe filters (Titan3/Target2 PTFE families), or PVDF variants rated for your solvent; confirm details on the specific SKU page.

Q5: How do I size syringe filters?
Pick diameter by volume/viscosity: 13 mm for small, clean samples; 17–25–30 mm for higher loads, viscous matrices, or when you want lower backpressure. Some Titan3/Target2 part numbers include a built-in prefilter for difficult samples.

Get a Quote from Iright

Tell us your application (cell culture media sterilization, HPLC sample prep, buffer clarification), target pore size, and solvent system, and we’ll match the correct membrane and format. Iright provides volume pricing, lead-time checks, and COA/documentation support across Rapid-Flow bottle-tops, Titan3/Target2/Choice syringe filters, and membrane discs.