Agilent, 5065-4463, ZORBAX 300SB-C8 3.5µm, 0.3 x 50

ZORBAX 300SB-C8 3.5µm, 0.3 x 50. A reversed-phase C8 HPLC column with fully porous ZORBAX particles. StableBond technology ensures stability at low pH.

Specifications:
Brand: ZORBAX 300SB-C8
Hardware: SS
Inner Diameter (ID): 0.3 mm
LC Platform: Stainless Steel
Length: 50 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: Monoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 3.5 µm
Particle Type: Fully Porous
Phase: 300SB-C8
Pore Size: 300 Å
Pressure Rating: 400 bar
Separation Mode: Reversed Phase
Shipping Solvent: Acetonitrile/Water
Technique: LCLC & LC/MS
UNSPSC Code: 41115709
pH Range: 1-8

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This high-performance reversed-phase HPLC column features a stable C8 bonded phase with a 3.5 micron particle size and dimensions of 0.3 mm internal diameter by 50 mm length. Engineered with a 300 Angstrom pore size, this column is optimized for the separation of peptides, small proteins, and other biomolecules, providing sharp peak resolution and high efficiency even with low sample volumes. Its robust end-capped stable bonding ensures reliable performance across a wide pH range, making it ideal for reproducible analyses in peptide mapping, proteomics, and biopharmaceutical research.

The column is particularly suited for use in high-throughput LC-MS workflows, especially for laboratories working with limited amounts of biological samples or requiring fast separations with strong sensitivity. It integrates seamlessly with Agilent 1100, 1200, and 1290 Infinity LC systems and is compatible with most UHPLC platforms designed for microbore columns. Researchers focused on peptide characterization, protein digest profiling, or complex biological matrices will benefit from the optimal combination of sample capacity, retention, and resolution. For broader method development, it complements Agilent's range of ZORBAX SB columns, offering flexibility in selectivity and pore size to accommodate a wide variety of analytical needs.