Agilent, 5065-9923, ZORBAX 300SB-C8, 3.5 µm, 0.075 x 50 mm

ZORBAX 300SB-C8, 3.5 µm, 0.075 x 50 mm. A reversed-phase C8 HPLC column with fully porous ZORBAX particles. StableBond technology ensures stability at low pH.

Specifications:
Brand: ZORBAX 300SB-C8
Hardware: SS
Inner Diameter (ID): 0.075 mm
LC Platform: Stainless Steel
Length: 50 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: Monoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 3.5 µm
Particle Type: Fully Porous
Phase: 300SB-C8
Pore Size: 300 Å
Pressure Rating: 400 bar
Separation Mode: Reversed Phase
Shipping Solvent: Acetonitrile/Water
Technique: LCLC & LC/MS
UNSPSC Code: 41115709
pH Range: 1-8

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The Agilent ZORBAX 300SB-C8, 3.5 µm, 0.075 x 50 mm column is a precision-engineered solution designed for high-performance liquid chromatography applications, particularly in proteomics and peptide mapping. Its 300 Å pore size and stable C8 bonded phase enable efficient separation of peptides, proteins, and other biomolecules with excellent peak shape and reproducibility. The narrow internal diameter and short column length support micro-flow and nano-LC workflows, making it ideal for sample-limited analyses or coupling with mass spectrometry for high-sensitivity detection.

This reversed-phase column exhibits outstanding stability at low pH, allowing compatibility with common acidic mobile phases used in biological sample analysis. It is widely used in pharmaceutical research, biotherapeutic characterization, and protein identification studies, where robust method performance and reproducibility are essential.

Engineered for compatibility with Agilent 1100, 1200, and 1290 Infinity LC systems, as well as other nano-LC platforms, this column integrates seamlessly into modern laboratory workflows. Related products, such as other ZORBAX StableBond columns in varying chemistries and dimensions, provide method development flexibility for diverse analytical needs. The ZORBAX 300SB-C8 column sets a high standard for reliability in sensitive biomolecule separations across life science research and development.