Agilent, 5190-2506, Agilent Bio SEC-3, 150Å, 7.8 x 300 mm, 3 µm

Agilent Bio SEC-3, 150Å, 7.8 x 300 mm, 3 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 0.5-150 kDa.

Specifications:
Brand: Agilent Bio SEC-3
Hardware: SS
Inner Diameter (ID): 7.8 mm
LC Platform: Stainless Steel
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 500 Da
Molecular Weight Upper Limit: 150000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 3 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 150 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-3, 150 Angstrom, 7.8 x 300 mm, 3 micron column is specifically engineered for high-resolution size exclusion chromatography (SEC) of biomolecules. Featuring a 150 Angstrom pore size and 3 micron particle size, this column delivers precise separation of proteins, peptides, and other biological macromolecules within a molecular weight range up to approximately 60 kDa. Its robust silica-based design ensures consistent performance, high efficiency, and reproducible results, making it ideal for analytical characterization of monoclonal antibodies, protein fragments, and biosimilars. The column is suitable for use in pharmaceutical development, quality control, and academic research settings requiring accurate molecular weight determination or assessment of sample aggregation and purity. Compatible with Agilent HPLC and UHPLC systems, including the Agilent 1260 Infinity II and 1290 Infinity II series, the column can also be integrated into other standard liquid chromatography platforms. For broader separation needs, complementary Bio SEC-3 columns are available in various pore sizes and dimensions. This solution offers researchers reliable performance and excellent reproducibility in protein analysis, supporting biomolecule characterization and method development for advanced protein therapeutics and related applications.