Agilent, 5190-2511, Agilent Bio SEC-3, 300Å, 7.8 x 300 mm, 3 µm

Agilent Bio SEC-3, 300Å, 7.8 x 300 mm, 3 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 5-1,250 kDa.

Specifications:
Brand: Agilent Bio SEC-3
Hardware: SS
Inner Diameter (ID): 7.8 mm
LC Platform: Stainless Steel
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 5000 Da
Molecular Weight Upper Limit: 1250000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 3 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 300 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-3 column, featuring a 300 Angstrom pore size with dimensions of 7.8 x 300 mm and 3 micron particle size, is designed for high-resolution size exclusion chromatography. This column is optimized for the separation and analysis of proteins, antibodies, and other biomolecules ranging from small peptides up to large proteins. Its high efficiency and excellent reproducibility make it ideal for characterizing molecular weight distributions and detecting protein aggregates in biopharmaceutical research and quality control.

This Bio SEC-3 column provides superior performance with both native and denatured samples, supporting a variety of buffer systems commonly used in biochemical and biophysical laboratories. It is fully compatible with Agilent HPLC and UHPLC systems, including models such as the 1260 Infinity and 1290 Infinity series, and can also be integrated with other major liquid chromatography platforms. The column is recommended for analytical applications such as process monitoring, product purity assessment, and formulation stability studies. To achieve enhanced analytical workflows, it can be used alongside Agilent’s comprehensive portfolio of SEC standards and sample preparation tools, offering a robust solution for accurate and reliable macromolecular analysis in pharmaceutical, biotechnology, and academic research settings.