Agilent, 5190-2512, Agilent Bio SEC-3, 300Å, 7.8 x 150 mm, 3 µm

Agilent Bio SEC-3, 300Å, 7.8 x 150 mm, 3 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 5-1,250 kDa.

Specifications:
Brand: Agilent Bio SEC-3
Hardware: SS
Inner Diameter (ID): 7.8 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 5000 Da
Molecular Weight Upper Limit: 1250000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 3 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 300 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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Designed for high-resolution size exclusion chromatography (SEC) of proteins and biopolymers, this column features a 300 Angstrom pore size, 7.8 mm internal diameter, and 150 mm length with 3 micron particle size. Its optimized silica-based particle technology delivers superior performance for molecular weight determination and aggregate analysis in research and quality control environments. Ideal for separating proteins and biomolecules within the 5,000 to 1,250,000 Dalton molecular weight range, it offers excellent efficiency, reproducibility, and low nonspecific adsorption for sensitive samples. The column is fully compatible with a wide range of Agilent HPLC and UHPLC systems, including the InfinityLab, 1100, 1200, and 1260 series, and can also be used with systems from other major instrument vendors. It is especially suited for applications in biopharmaceutical characterization, including monoclonal antibody analysis, vaccine research, and enzyme studies. Recommended usage includes screening for aggregates and fragments, monitoring product stability, and assessing purity. The column works seamlessly with Agilent SEC mobile phases and is supported by a comprehensive portfolio of related bioanalytical columns to ensure robust and reproducible results for both routine and advanced biomolecular analyses.