Agilent, 5190-2513, Agilent Bio SEC-3, 300Å, 4.6 x 300 mm, 3 µm

Agilent Bio SEC-3, 300Å, 4.6 x 300 mm, 3 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 5-1,250 kDa.

Specifications:
Brand: Agilent Bio SEC-3
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 5000 Da
Molecular Weight Upper Limit: 1250000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 3 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 300 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

**********The following product description is generated by AI and is for reference only. If you have any questions, please contact customer service.**********
Designed for high-resolution size exclusion chromatography, this silica-based column features a 300 Angstrom pore size, 4.6 x 300 mm dimensions, and 3 micron particle size, ensuring precise separation of biomolecules such as proteins, peptides, and other macromolecules. Offering excellent reproducibility and minimal non-specific interactions, it is ideal for researchers requiring accurate molecular weight determinations or aggregate analysis in biotechnology and pharmaceutical development.

The column's optimized design provides sharp peak resolution and fast analysis times, supporting applications ranging from protein purity assessment to the characterization of antibody-drug conjugates and biotherapeutics. Its robust construction is suitable for frequent use in demanding laboratory environments, while the silica matrix ensures extended column lifetime under typical aqueous mobile phase conditions.

Compatible with Agilent HPLC and UHPLC systems, as well as many standard chromatographic platforms, this column integrates seamlessly into existing laboratory workflows. It can be used alongside Agilent's suite of SEC calibration standards and data analysis solutions, providing a comprehensive solution for size exclusion applications. This configured column supports a broad spectrum of analytical needs in research, quality control, and process monitoring, empowering scientists to achieve reliable and reproducible results for biomolecule characterization.