Agilent, 5190-2514, Agilent Bio SEC-3, 300Å, 4.6 x 150 mm, 3 µm

Agilent Bio SEC-3, 300Å, 4.6 x 150 mm, 3 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 5-1,250 kDa.

Specifications:
Brand: Agilent Bio SEC-3
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 5000 Da
Molecular Weight Upper Limit: 1250000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 3 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 300 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-3, with 300 Angstrom pore size, a 4.6 x 150 mm column dimension, and 3 micron particle size, is designed for high-performance size exclusion chromatography of biological macromolecules. This column enables precise molecular size separation and characterization of proteins, antibodies, and other biomolecules ranging from small peptides to larger protein complexes. Its uniform pore structure and optimized silica support ensure excellent resolution, reproducibility, and minimal nonspecific interactions, making it ideal for protein aggregation analysis, purity determination, and molecular weight estimation in pharmaceutical, biotechnology, and academic laboratories.

Optimized for advanced liquid chromatography systems, the column performs seamlessly with Agilent HPLC and UHPLC platforms, as well as other major brands. It is compatible with both aqueous and selected organic mobile phases, supporting a wide range of biotherapeutic analysis workflows. This column is particularly suited for applications including quality control, process development, and formulation studies. Researchers can also integrate it with Agilent’s comprehensive suite of detectors, such as UV, refractive index, and light scattering detectors, for enhanced data quality and flexibility. This product is part of the Agilent Bio SEC column portfolio, providing scalability and method transferability across different analysis needs.