Agilent, 5190-2516, Agilent Bio SEC-5, 100Å, 7.8 x 300 mm, 5 µm

Agilent Bio SEC-5, 100Å, 7.8 x 300 mm, 5 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 0.1-100 kDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 7.8 mm
LC Platform: Stainless Steel
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 100 Da
Molecular Weight Upper Limit: 100000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 100 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-5 column, with 100 angstrom pore size, 7.8 x 300 mm dimensions, and 5 micron particle size, is engineered for high-resolution size exclusion chromatography of biomolecules. This column delivers precise molecular weight determination and efficient separation of proteins, peptides, and other biological macromolecules in both research and quality control laboratories. Its stable silica-based packing and proprietary bonding technology ensure optimal peak symmetry, minimal non-specific interactions, and reproducible results with every run.

Ideal for use with HPLC and UHPLC systems, the column is compatible with a range of Agilent instrumentation such as the 1200 Infinity II and 1260 Infinity II series. It is particularly suited for method development, protein purity analysis, aggregate monitoring, and molecular size profiling in biopharmaceutical, academic, and clinical research settings. The robust performance supports a wide pH range and is compatible with aqueous and organic solvent systems, facilitating flexible method optimization.

For expanded analytical workflows, it can be used alongside other pore-sized columns in the Agilent Bio SEC-5 series to achieve comprehensive size-based separations across a range of biotherapeutics and research-grade proteins. This product underpins reliable, high-throughput analysis for critical biomolecule characterization tasks.