Agilent, 5190-2519, Agilent Bio SEC-5, 100Å, 4.6 x 150 mm, 5 µm

Agilent Bio SEC-5, 100Å, 4.6 x 150 mm, 5 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 0.1-100 kDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 100 Da
Molecular Weight Upper Limit: 100000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 100 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-5, 100 Angstrom, 4.6 x 150 mm, 5 micron column is purpose-designed for high-resolution size exclusion chromatography of biological macromolecules such as proteins, peptides, and nucleic acids. Crafted with a proprietary silica-based matrix and a 100 Angstrom pore size, this column delivers accurate molecular size separation while minimizing sample interaction with the stationary phase. The 4.6 mm internal diameter and 150 mm length offer a balanced platform for both analytical throughput and optimal resolution, suiting workflows in biopharmaceutical analysis, protein aggregation studies, and quality control environments.

Its 5 micron particle size supports efficient flow rates and reproducible performance, which makes it ideal for routine laboratory applications and advanced research. The column is fully compatible with most standard HPLC and UHPLC systems, including Agilent’s InfinityLab and 1260 Infinity II LC series, as well as systems from other major manufacturers. It is recommended for use with aqueous mobile phases and can be paired with Agilent SEC calibration standards for precise molecular weight determination. This versatile column supports applications in drug development, biologics characterization, and academic research requiring reliable, high-efficiency biomolecule separation.