Agilent, 5190-2537, Agilent Bio SEC-5, 1000Å, 7.8 x 150 mm, 5 µm

Agilent Bio SEC-5, 1000Å, 7.8 x 150 mm, 5 µm. A size exclusion column for aggregate analysis of large biological molecules such as adeno associated virus and virus-like particles. Resolving range 15 kDa - 5 MDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 7.8 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 50000 Da
Molecular Weight Upper Limit: 7500000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 1000 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-5 column, featuring 1000Å pore size, 7.8 x 150 mm dimensions, and 5 µm particle size, is engineered for advanced size exclusion chromatography applications involving biomolecules such as proteins, peptides, and biotherapeutics. This silica-based column provides high-resolution separation, enabling precise molecular weight determination and aggregate analysis in complex samples. Its robust design delivers reproducible results and extended column lifetime, even under demanding analytical conditions.

Optimized for aqueous mobile phases, this column is well-suited for use in protein characterization, quality control, and formulation studies in pharmaceutical, biotechnology, and academic research environments. Researchers frequently employ this column for the analysis of monoclonal antibodies, enzyme conjugates, and vaccine components, benefiting from its low nonspecific adsorption and minimal sample interaction.

The Bio SEC-5 column seamlessly integrates with Agilent HPLC and UHPLC systems, including the 1260 Infinity and 1290 Infinity LC series. It is also compatible with systems from other leading manufacturers, supporting versatile laboratory workflows. For broader analytical needs, this column complements other Agilent Bio SEC columns of varying pore sizes, allowing comprehensive analysis of biomolecules across a wide molecular weight range.