Agilent, 5190-2539, Agilent Bio SEC-5, 1000Å, 4.6 x 150 mm, 5 µm

Agilent Bio SEC-5, 1000Å, 4.6 x 150 mm, 5 µm. A size exclusion column for aggregate analysis of large biological molecules such as adeno associated virus and virus-like particles. Resolving range 15 kDa - 5 MDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 50000 Da
Molecular Weight Upper Limit: 7500000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 1000 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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Designed for high-performance size exclusion chromatography, this column features 5 µm particles and a 1000 angstrom pore size, making it ideal for the analysis of biomolecules such as proteins, peptides, and other macromolecules. With dimensions of 4.6 x 150 mm, it delivers excellent resolution and reproducibility for molecular weight determination, aggregate analysis, and oligomer separation. The robust silica-based stationary phase ensures chemical stability and minimal nonspecific interactions, supporting accurate and repeatable results in both research and quality control laboratories.

Optimized for use with a wide range of HPLC and UHPLC systems, including Agilent 1100, 1200, 1260, and 1290 Infinity series, this column is compatible with commonly used aqueous mobile phases. It is particularly suitable for biopharmaceutical development, protein purity assessment, and characterization of antibodies and other biological therapeutics. The column is also an excellent choice for academic and industrial labs focused on protein engineering, formulation, or aggregate studies. For extended analytical needs, related SEC columns with different pore sizes and dimensions are available, enabling method scalability and transferability across various applications in life science research.