Agilent, 5190-6850, Agilent Bio SEC-3 100Å, 21.2 x 300 mm, 3 µm

Agilent Bio SEC-3 100Å, 21.2 x 300 mm, 3 µm. A preparative scale size exclusion column for the purification of biological samples. Resolving range 0.1-100 kDa.

Specifications:
Brand: Agilent Bio SEC-3
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 100 Da
Molecular Weight Upper Limit: 100000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 3 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 100 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-8.5

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The Agilent Bio SEC-3 100 Angstrom column, sized at 21.2 x 300 mm with a 3 micron particle size, is designed for high-resolution size exclusion chromatography (SEC) of biomolecules such as proteins, peptides, and other biological macromolecules. Optimized for analytical and semi-preparative separation, this column delivers precise molecular size determination and excellent reproducibility, making it ideal for applications in protein aggregation studies, purity assessments, and characterization of biopharmaceuticals. The stationary phase features a robust silica matrix and a carefully engineered pore size, supporting efficient analysis of molecules with hydrodynamic radii relevant to antibody, fragment, and peptidic therapeutics. Compatible with Agilent's full range of HPLC and UHPLC systems, this SEC column integrates seamlessly with detectors such as multi-angle light scattering, refractive index, and UV to support method development and quality control workflows. Its use is also recommended in conjunction with Agilent SEC standards and columns of varying pore sizes for comprehensive biomolecule analysis. Quality manufacturing ensures batch-to-batch consistency, making this column a trusted choice for pharmaceutical research, biotechnology, and academic laboratories seeking reliable size-based separation.