Agilent, 5190-6852, Agilent Bio SEC-3, 300Å, 21.2 x 300 mm, 3 µm

Agilent Bio SEC-3, 300Å, 21.2 x 300 mm, 3 µm. A preparative scale size exclusion column for the purification of biological samples. Resolving range 5-1,250 kDa.

Specifications:
Brand: Agilent Bio SEC-3
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 5000 Da
Molecular Weight Upper Limit: 1250000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 3 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 300 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-8.5

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Designed for high-resolution biomolecular size analysis, this advanced size exclusion chromatography column features a 300 ångström pore size and 3 micron particle size, housed in a 21.2 x 300 mm format. It delivers precise and reproducible separation of proteins, antibodies, and biopolymers with molecular weights ranging from approximately 10 kDa to 1,500 kDa. Its superfine particle size ensures outstanding resolution and peak symmetry, making it ideal for accurate characterization of aggregates, fragments, and monomer content in research and quality control environments.

This column is well suited for both analytical and semi-preparative workflows in fields such as biopharmaceutical development, protein engineering, and academic research. It supports fast analysis times and robust performance under a variety of high-performance liquid chromatography systems, including Agilent 1260 Infinity II and 1290 Infinity II platforms. The column is fully compatible with commonly used aqueous mobile phases, buffers, and conditions required for sensitive biomolecule separations. For streamlined laboratory workflows, it may be paired with a range of Agilent bio-inert accessories and detectors to ensure sample integrity and optimal detection. Its durable construction and consistent results make it a reliable choice for routine and challenging biomolecular characterization tasks.