Agilent, 5190-6856, Agilent Bio SEC-3, 300Å, 21.2 x 50 mm, 3 µm

Agilent Bio SEC-3, 300Å, 21.2 x 50 mm, 3 µm. A preparative scale size exclusion column for the purification of biological samples. Resolving range 5-1,250 kDa.

Specifications:
Brand: Agilent Bio SEC-3
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 50 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 5000 Da
Molecular Weight Upper Limit: 1250000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 3 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 300 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-8.5

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The Agilent Bio SEC-3, 300Å, 21.2 x 50 mm, 3 µm column delivers high-resolution size exclusion chromatography for the analysis and purification of biomolecules such as proteins, peptides, and biotherapeutics. Engineered with a uniform, narrow particle size distribution and precise 300 angstrom pore size, this column enables accurate molecular size determination, aggregate analysis, and quantitation of high- and low-molecular-weight species.

Ideal for laboratories engaged in biopharmaceutical research, quality control, or academic studies, the column provides superior separation efficiency and reproducibility for critical applications like protein aggregation studies, monoclonal antibody characterization, and formulation development. Its robust silica-based construction supports rapid analysis while minimizing carryover and ensuring long column lifespan.

Compatible with Agilent HPLC and UHPLC systems as well as a broad range of third-party chromatographic platforms, this column integrates seamlessly into existing workflows. It pairs effectively with Agilent’s autosamplers, detectors, and software solutions, enabling streamlined data acquisition and analysis. For optimized results, researchers may also consider using matched SEC guard columns or other pore size variants within the Bio SEC-3 series depending on analyte size range and specific separation needs.