Agilent, 5190-6863, Agilent Bio SEC-5, 100Å, 21.2 x 300 mm, 5 µm

Agilent Bio SEC-5, 100Å, 21.2 x 300 mm, 5 µm. A preparative scale size exclusion column for the purification of biological samples. Resolving range 0.1-100 kDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 100 Da
Molecular Weight Upper Limit: 100000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 100 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-8.5

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The Agilent Bio SEC-5 column with 100 angstrom pore size and dimensions of 21.2 x 300 millimeters, featuring 5 micron particle size, is engineered for high-resolution size exclusion chromatography (SEC) of biomolecules, including proteins, peptides, and nucleic acids. Its silica-based, hydrophilic packing ensures minimal nonspecific binding and excellent protein recovery, making it ideal for the analysis and purification of complex biological samples. The column supports routine quality control and characterization of monoclonal antibodies, aggregation studies, and quantitation of molecular weight variants in biopharmaceutical research and manufacturing. Designed for compatibility with Agilent HPLC and UHPLC systems, this column integrates seamlessly into workflows requiring scalable analysis, with performance suitable for both analytical and preparative applications. Additionally, it is well-suited for use with other major liquid chromatography platforms that accommodate standard column formats. The Bio SEC-5’s robust mechanical stability enables efficient separation with consistent peak symmetry and reliable reproducibility over extended use. For optimal results, it can be paired with Agilent SEC calibration standards and solvents, ensuring accurate molecular weight determinations. This product supports scientists and researchers in delivering confident, reproducible data in the evolving field of protein therapeutics and biomolecular research.