Agilent, 5190-6865, Agilent Bio SEC-5, 300Å, 21.2 x 300 mm, 5 µm

Agilent Bio SEC-5, 300Å, 21.2 x 300 mm, 5 µm. A preparative scale size exclusion column for the purification of biological samples. Resolving range 5-1,250 kDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 5000 Da
Molecular Weight Upper Limit: 1250000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 300 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-8.5

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The Agilent Bio SEC-5 300A column, with dimensions of 21.2 x 300 mm and a 5 micron particle size, is specially engineered for high-resolution size exclusion chromatography of biomolecules. Featuring a robust hydrophilic bonded phase and 300 angstrom pore size, this column ensures efficient separation of large proteins, monoclonal antibodies, aggregates, and other biotherapeutics, supporting both analytical and preparative workflows. Its large bore diameter allows for increased sample loading and rapid throughput, ideal for process scale-up, purification, and quality control tasks in biopharmaceutical research.

This column is compatible with Agilent high-performance liquid chromatography systems, such as the 1260 Infinity and 1290 Infinity LC series, and integrates seamlessly with other commonly used SEC instruments. It is also suitable for pairing with Agilent’s range of bio-inert HPLC modules to minimize sample interaction and maximize biomolecule recovery. Typical research applications include protein characterization, aggregate quantification, and molecular weight determination of complex biological samples. Designed for reliability and reproducibility, this column is an effective solution for rigorous protein analysis and purification in academic, biotech, and pharmaceutical laboratories. For optimal results, users may consider integrating with Agilent certified SEC standards and related bioseparation columns.