Agilent, 5190-6869, Agilent Bio SEC-5, 100Å, 21.2 x 50 mm, 5 µm

Agilent Bio SEC-5, 100Å, 21.2 x 50 mm, 5 µm. A preparative scale size exclusion column for the purification of biological samples. Resolving range 0.1-100 kDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 50 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 100 Da
Molecular Weight Upper Limit: 100000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 100 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-8.5

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The Agilent Bio SEC-5 column, featuring 100 Angstrom pore size, 21.2 x 50 mm dimensions, and 5 micron particle size, is designed for high-resolution size exclusion chromatography of biological macromolecules. This column delivers accurate and reproducible separation of proteins, antibodies, and other biomolecules by molecular size, making it ideal for aggregate analysis, molecular weight determination, and biomolecule purity assessment. With a robust silica-based matrix and optimized surface chemistry, it ensures minimal non-specific interactions and reliable performance with both aqueous and organic mobile phases.

This column is particularly suited for the analysis of monoclonal antibodies, therapeutic proteins, and protein fragments, serving applications in biopharmaceutical development, quality control, and academic research. It is compatible with Agilent HPLC and UHPLC systems, including the 1260 Infinity and 1290 Infinity series, and integrates seamlessly with popular detectors such as UV, fluorescence, and refractive index for comprehensive analytical workflows. For researchers seeking to tailor their separations, the Bio SEC-5 range offers various pore sizes and dimensions. This product is also cross-compatible with systems from other leading analytical instrument manufacturers, supporting flexibility in laboratory integration and method development.