Agilent, 5190-6873, Agilent Bio SEC-5, 1000Å, 21.2 x 50 mm, 5 µm

Agilent Bio SEC-5, 1000Å, 21.2 x 50 mm, 5 µm. A preparative scale size exclusion column for the purification of very large biological samples such as virus like particles. Resolving range 50 kDa - 7.5 MDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 50 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 50000 Da
Molecular Weight Upper Limit: 7500000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 1000 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-8.5

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The Agilent Bio SEC-5 column, featuring 1000 angstrom pore size and 5 micron particle size in a 21.2 by 50 millimeter format, is designed for high-resolution size exclusion chromatography of biological macromolecules. It delivers accurate molecular weight separation and superior recovery of proteins, antibodies, and other biomolecules, accommodating a wide range of sample sizes in both analytical and preparative scales. Ideal for the characterization of aggregates, protein purity analysis, and determination of molecular weight distributions, the column is particularly suited for research and quality control in biopharmaceutical development. The robust silica-based particles and precisely engineered pore structure enable consistent performance and excellent reproducibility. Compatible with Agilent 1200 Series, 1260 Infinity, and 1290 Infinity LC systems, as well as most standard HPLC or bioLC systems, the column integrates seamlessly into existing laboratory workflows. For enhanced results, it can be paired with Agilent’s comprehensive range of SEC standards and mobile phases. This product is recommended for applications requiring reliable, reproducible macromolecule separations, supporting both routine sample analysis and critical research in protein therapeutics and biotechnology laboratories.