Agilent, 5190-6881, Agilent Bio WCX, NP5, 21.2 x 250 mm, 5 µm

Agilent Bio WCX, NP5, 21.2 x 250 mm, 5 µm. A weak cation exchange prep sized HPLC column with nonporous particles. Particle size is 5 μm. Nonporous particles are best for efficient mass transfer and high resolution separations.

Specifications:
Brand: Agilent Bio WCX
Column Material: Stainless Steel
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 250 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: Charge Variant AnalysisPeptidesProteins
Particle Size: 5 µm
Particle Type: Non-porous
Phase: WCX
Pressure Rating: 413 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-12

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Designed for robust and reliable bioseparations, this weak cation exchange preparative column features a 21.2 x 250 mm dimension and 5 µm particle size, delivering exceptional resolution and capacity for protein and peptide purification. Its high-performance polymethacrylate-based resin ensures consistent results, making it an excellent choice for both research and process scale applications in biotechnology and pharmaceutical development.

Engineered for use with Agilent HPLC and preparative LC systems, the column enables efficient scale-up from analytical to preparative workflows. It supports the purification of monoclonal antibodies, recombinant proteins, and other biologically relevant cationic biomolecules, demonstrating outstanding stability under various pH and salt conditions. Users benefit from fast equilibration and cleaning, streamlined method development, and compatibility with automated fraction collection in large-scale operations.

This column integrates seamlessly with related Agilent consumables and detectors, supporting comprehensive protein characterization workflows. It is suitable for labs involved in downstream processing, method optimization, and validation, as well as those performing quality control and preclinical research. Reliable performance, easy method transfer, and an industry-leading lifetime make this column an essential tool for advanced bioseparation requirements.