Agilent, 773995-902, ZORBAX Extend 300 C18, 4.6 x 150 mm, 5 µm

ZORBAX Extend 300 C18, 4.6 x 150 mm, 5 µm. A reversed-phase C18 HPLC column with fully porous ZORBAX particles. The 300-Extend C18 chemistry incorporates a unique bidentate silane which allows the column to withstand pH up to 11.5.

Specifications:
Brand: ZORBAX
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 40 °C at pH 8-11.560 °C at pH 6-8
Part Number Application Domain: Biopharma
Part Number Application: Monoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: Extend-C18
Pore Size: 300 Å
Pressure Rating: 400 bar
Separation Mode: Reversed Phase
Shipping Solvent: Acetonitrile/Water
Technique: LCLC & LC/MS
UNSPSC Code: 41115709
pH Range: 2-11

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The ZORBAX Extend 300 C18 column offers reliable performance for reversed-phase high-performance liquid chromatography applications, especially for the separation of proteins, peptides, and other biomolecules. Featuring a 4.6 x 150 mm dimension and 5 micron particle size, this column provides high resolution and capacity, making it suitable for analytical and preparative workflows in proteomics, pharmaceutical development, and biochemistry research.

Its proprietary extended pH stability allows operation from pH 2 to 11, enabling the analysis of samples that require harsh or basic mobile phases, as well as prolonged column lifetime for challenging analyses. The 300 angstrom pore size efficiently accommodates larger molecules, improving peak shapes and recoveries for biomolecules and synthetic peptides. The robust C18 bonded phase ensures consistent, reproducible retention and separation of hydrophobic analytes.

This column is compatible with Agilent HPLC and UHPLC systems, including 1100, 1200, 1260, and 1290 Infinity Series, as well as most other standard liquid chromatography instruments. It integrates seamlessly with a range of ZORBAX columns to provide method flexibility and scalability. The ZORBAX Extend 300 C18 column is an optimal choice for researchers requiring robust and high-efficiency separations across a variety of life science and pharmaceutical applications.